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Objective To examine the barbiturate infusion for intractable intracranial hypertension and its effect on brain tissue oxygen ( PbtO2 ). Method 60 patients with intractable intracranial hypertension were divided into tow groups, experimental group and control group. PbtO2 and intracranial pressure (ICP) were continuous monitored. Follow-up was more than 3 months and the prognosis was compared between two groups. Results When pentobarbital administration began, the mean PbtO2 [ 72 h: (26. 7 ±6. 7)mmHg] at 24h, 48h and 72h in experimental group were significantly higher than that of control group [ 72 h: ( 21. 1 ± 7. 2) mmHg ] ( P < 0. 05 ). The mean ICP [ 5 d: (2. 48 ± 1.11 ) kPa ] in the third and fifth day of experimental group were lower than that of control group [ 5 d: (3. 12 ± 1.09 ) kPa ]. Prognosis of pentobarbital group was better than control group( P < 0. 05 ). Conclusions Pentobarbital can effectively reduce intracranial pressure in patients with refractory intracranial hypertension and improve brain tissue oxygen. PbtO2 is an ideal monitoring marker, and it can predict prognosis to a certain extent.
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@# Objective To investigate the effect of open nursing management on hospital rehabilitation of patients with schizophrenia.MethodsThe outcome of group A,which included 113 patients in open nursing management,and group B,which included 141 patients in close nursing management at the same time,were evaluated with the Brief Psychiatric Rating Scale(BPRS)and the Nurses' Observation Scale for Impatient Evaluation(NOSIE),etc.ResultsThe difference between the two groups in such as the time of symptoms improved,drugs administration,effective rates were not significant(P>0.05).The compliance of medicine,social capability and personal sanitation of group A were improved compared with those in group B(P<0.05),while the time stay in hospital obviously shortened(P<0.01).ConclusionThe open nursing management is helpful to improve the rehabilitation and shorten the time stay in hospital of patients with schizophrenia.
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<p><b>OBJECTIVE</b>To investigate the possibility of using melanoma antigen-1 (MAGE-1) peptide as a tumor vaccine to treat hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>The expressions of MAGE-1 in 8 HCC cell lines and in liver cancer tissue from a patient were detected using RT-PCR. The type of human leucocyte antigen I(HLA I) of both 8 HCC cell lines and peripheral blood mononuclear cells of the patient was detected using a microcytotoxicity method to screen out target cell lines for the cytotoxicity assay. Peripheral blood mononuclear cells from the HCC patient pulsed with an MAGE-1 peptide (NYKCRFPEI) were used as antigen presenting cells. Autogenous peripheral blood mononuclear cells were stimulated with antigen presenting cells every 7 days for 4 times to elicit cytotoxic T lymphocytes. The phenotype of effector cells was analyzed using flow cytometry. The cytotoxicity of effector cells was detected with a lactate dehydrogenase releasing assay.</p><p><b>RESULTS</b>The expressions of both MAGE-1 and HLA-A24 were detected in BEL7405 cell line which were used as the positive target cell line in the cytotoxicity assay. The expression of MAGE-1 alone was detected in HLE, BEL7402, BEL7404, QGY7703 and SMMC7721 cell lines, and the expression of neither MAGE-1 nor HLA-A24 was shown in QGY 7701 and HpG2 cell lines. The last 7 cell lines could be used as negative target cell lines in the cytotoxicity assay. Peripheral blood mononuclear cells expanded 32 folds during 28-day culture. The ratio of CD3(+) T cells increased by 16% (from 54% to 70%), and the ratio of CD8(+) T cells increased by 20% (from 36% to 56%) during 28-day culture. When the ratio of effector cells to target cells was 10:1, effector cells exhibited 62.5% cytotoxicity against autogenous lymphoblasts pulsed with the peptide (NYKCRFPEI) of MAGE-1 antigen, 40.25% cytotoxicity against BEL7405 cells, compared with 17.88% cytolysis observed against autogenous lymphoblasts, 19.55% against HLE cells, and 1.6% against QGY7701 cells. When the ratio of effector cells to target cells was 3.3:1, the cytotoxicity of effector cells against the peptide pulsed autogenous lymphoblasts was 53.6%, which was much higher against autogenous lymphoblasts, HLE cells and QGY7701 cells at 15.6%, 13% and 1%, respectively.</p><p><b>CONCLUSION</b>The results demonstrate that cytotoxic T lymphocytes with the ability to specifically lyse target cells expressing both MAGE-1 and HLA-A24 could be successfully induced by the MAGE-1 peptide NYKCRFPEI in vitro. This indicates that a good result might be anticipated if this peptide is used as a tumor vaccine to treat HLA-A24 HCC patients.</p>
Subject(s)
Adult , Humans , Male , Antigens, Neoplasm , Cancer Vaccines , Allergy and Immunology , Carcinoma, Hepatocellular , Allergy and Immunology , HLA-A Antigens , HLA-A24 Antigen , Liver Neoplasms , Allergy and Immunology , Melanoma-Specific Antigens , Neoplasm Proteins , Genetics , Allergy and Immunology , RNA, Messenger , T-Lymphocytes, Cytotoxic , Allergy and Immunology , Tumor Cells, CulturedABSTRACT
Object To investigate the effect of cistanche glycosides (GCs) on immune functions and 30 d survival rate of 60 Co irradiated mice Methods Mice were given GCs both before and after 60 Co irradiation at different doses, and the changes of their immunological functions observed by routine immunopharmacologic methods Results When irradiated at doses of 2, 4, and 7 Gy with 60 Co, the humoral, cellular and non specific immunity, and 30 d survival rate were all decreased (P